Sample Should be collected B/w 11-13.6 Weeks of Pregnancy, Patient Weight , Height, DOB, Left hand Systolic/ Diastolic BP and Right hand Systolic/ Diastolic BP required Ultrasound report with CRL (Crown rump length), NB (Nasal Bone), NT (Nuchal Translucency) and (DVPi) Ductus venosus pulsatility index is required along with serum Sample