Preparation of PPP: 12 -14 Hrs. (Overnight) fasting is preferred. Centrifuge the Blood sample at 3rpm for 1 minutes within 1 hr. of collectionto separate the plasma. Transfer the Plasma obtained to a clean plastic tube using a plastic tipped pipette avoiding buffy coat & centrifuge it again at 37 rpm & finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <1, cells/cu. mm.Duly filled Coagulation Requisition Form (Form 15) is mandatory.